SELDI-TOF MS Analysis of Hepatocellular Carcinoma in an Australian Cohort
Journal of Surgical Research, Volume 238, June 2019, Pages 127-136.
Steven M Schlichtemeier, Christopher B Nahm, Aiqun Xue, Anthony J Gill, Ross C Smith, Thomas J Hugh
Hepatocellular carcinoma (HCC) is a common cause of cancer death worldwide. Resection offers the best chance of long-term survival, but a consistent adverse prognostic factor is the presence of microvascular invasion (MVI). In this study, surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS), a high throughput method of analyzing complex samples, was used to explore differentially expressed proteins between HCC and adjacent nontumour liver tissue (ANLT). A panel of four proteins was identified with excellent discriminative capacity between HCC and ANLT. Of these, m/z 9961 was the only protein significantly associated with a known poor prognostic factor (presence of MVI) and survival (shorter disease-free survival).
Reciprocal Interplay of miR-497 and MALAT1 Promotes Tumourigenesis of Adrenocortical Cancer
Endocr Relat Cancer. 2019 Jul; 26(7): 677-688.
Nunki Hassan, Jing Ting Zhao, Anthony Glover, Bruce G Robinson, Stan B Sidhu
Adrenocortical carcinoma (ACC) has high recurrence rates and poor prognosis with limited response to conventional cancer therapy. Recent contributions of high-throughput transcriptomic profiling identified microRNA-497 (miR-497) as significantly underexpressed, while lncRNA MALAT1 (metastasis-associated lung adenocarcinoma transcript 1) as overexpressed in ACC. The aim was to investigate the interaction of miR-497 and MALAT1 in ACC and its functional roles in the process of tumourigenesis. In this study, it was demonstrated that miR-497 post- transcriptionally repressed MALAT1 while MALAT1 also competes for miR-497 binding to its molecular target, EIF4E (eukaryotic translation initiation factor 4E). We showed that overexpression of miR-497 and silencing of MALAT1 suppressed cellular proliferation and induced cell cycle arrest through downregulation of EIF4E expression.
Furthermore, MALAT1 directly binds to SFPQ (splicing factor proline and glutamine rich) protein, indicating its multifaceted roles in ACC pathophysiology. This is the first study to identify the feedback axis of miR-497-MALAT1/ EIF4E in ACC tumourigenesis, providing novel insights into the molecular functions of noncoding RNAs in ACC.
Glioblastoma Recurrence Correlates With Increased APE1 and Polarization Toward an Immuno-Suppressive Microenvironment
Front Oncol. 2018; 8: 314.
Amanda L Hudson, Nicole R Parker, Peter Khong, Jonathon F Parkinson, Trisha Dwight, Rowan J Ikin, Ying Zhu, Jason Chen, Helen R Wheeler, and Viive M Howell
While treatment with surgery, radiotherapy and/or chemotherapy may prolong life for patients with glioblastoma, recurrence is inevitable. What is still being discovered is how much these treatments and recurrence of disease affect the molecular profiles of these tumors and how these tumors adapt to withstand these treatment pressures. Overall, our findings highlight the ability of glioblastomas to evade not only the toxic onslaught of therapy but also to evade the immune system; and suggest that a two-pronged approach may be needed, one treatment targeting the treatment resistance mechanism and another targeting the immune suppression.
Novel serum protein biomarker panel revealed by mass spectrometry and its prognostic value in breast cancer
Breast Cancer Res. 2014; 16(3): R63.
Liping Chung, Katrina Moore, Leo Phillips, Frances M Boyle, Deborah J Marsh, and Robert C Baxter
Serum profiling using proteomic techniques has great potential to detect biomarkers that might improve diagnosis and predict outcome for breast cancer patients (BC). This study used surface-enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry (MS) to identify differentially expressed proteins in sera from BC and healthy volunteers (HV), with the goal of developing a new prognostic biomarker panel. This study has revealed five serum proteins which, in combination, can distinguish between serum from women with breast cancer and healthy control subjects with high sensitivity and specificity. The five-protein panel significantly predicts recurrence-free survival in women with ER-negative tumors and may have value in the management of these patients.
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